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Clinical Chemistry 27: 526-529, 1981;
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Clinical Chemistry, Vol 27, 526-529, Copyright © 1981 by American Association for Clinical Chemistry

Esterolytic method for determination of heparin in plasma

GF Kapke, RD Feld, DL Witte and WG Owen

This assay for heparin is based on the heparin-accelerated rate of alpha-thrombin III. The rate or product formation from the residual active thrombin is inversely proportional to plasma heparin content. The assay can be performed manually, but our results were obtained with a discrete analyzer, the ABA-100. The assay is insensitive to concentrations of antithrombin III in plasma. Precision studies gave CVs of less than 10%. This assay was compared to the Dade Protopath heparin assay and a correlation coefficient of 0.90 was obtained (n = 62). The correlation between activated partial thromboplastin times and heparin concentrations (r = 0.67) was calculated frm results on 78 plasma specimens from 10 patients.




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Copyright © 1981 by the American Association for Clinical Chemistry.