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Clinical Chemistry 27: 553-555, 1981;
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Clinical Chemistry, Vol 27, 553-555, Copyright © 1981 by American Association for Clinical Chemistry

Evaluation of pteroylglutamic acid and n-5-methyltetrahydrofolic acid reference standards in a new "No-Boil" radioassay for serum folate

RA Theobald, M Batchelder and MF Sturgeon

Serum folate, pteroylglutamic acid standards, and N-5- methyltetrahydrofolate standards extract differently during sample preparation for radiometric assay. B/B0 values for each material were determined before and after an alkaline extraction procedure. Serum folate most closely resembled N-5-methyltetrahydrofolate standards in that it exhibited a slightly greater ability to displace iodinated folate derivative in competition for beta-lactoglobulin binder. Pteroylglutamic acid standards displaced folate derivative less well, so that serum folate values were lower than when N-5- methyltetrahydrofolate was used as the standard material.




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Copyright © 1981 by the American Association for Clinical Chemistry.