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Clinical Chemistry 27: 603-604, 1981;
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Clinical Chemistry, Vol 27, 603-604, Copyright © 1981 by American Association for Clinical Chemistry

Positive interference by danazol in a testosterone radioimmunoassay kit procedure

AM Sharp, IS Fraser, S Robertson and JR Turtle

We describe a problem in measuring serum testosterone concentrations in the serum of women who are taking danazol. The Diagnostic Products Corporation testosterone radioimmunoassay gives very high apparent testosterone concentrations in these women and the value obtained depends on the volume of serum extracted per assay tube. Chromatography of the serum extracts on Sephadex LH-20, but not on Celite, before radioimmunoassay reduced these high apparent testosterone concentrations to less than that found in normal women.




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Copyright © 1981 by the American Association for Clinical Chemistry.