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Clinical Chemistry 27: 607-609, 1981;
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Clinical Chemistry, Vol 27, 607-609, Copyright © 1981 by American Association for Clinical Chemistry

Insulin degradation by human erythrocyte lysates

SG Nerurkar and KK Gambhir

In vitro hemolysates of isolated human erythrocytes degrade 125I- labeled insulin. Ten- to 100-fold dilutions of the hemolysate give a proportionately decreased degradation of 125I-labeled insulin at 37 degrees C, while dilutions of up to eightfold do not. Like the control, diluted "Buffer G" containing 5 mmol/L Tris and 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid buffer alone, more than 500-fold dilutions of the hemolysate or boiled hemolysate (in buffer) caused negligible (less than 1%) degradation of the labeled insulin. We conclude that accurate insulin-binding data during erythrocyte insulin radioreceptor assay under optimum conditions (Clin. Chem. 23: 1590- 1595, 1977) depend on avoiding or minimizing hemolysis.


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P. H. Hinderling
Red Blood Cells: A Neglected Compartment in Pharmacokinetics and Pharmacodynamics
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Copyright © 1981 by the American Association for Clinical Chemistry.