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Clinical Chemistry 27: 614-619, 1981;
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Clinical Chemistry, Vol 27, 614-619, Copyright © 1981 by American Association for Clinical Chemistry

Preincubation of serum aspartate aminotransferase with pyridoxal 5'- phosphate in the SMAC: comparison with revised DuPont aca method and recommended IFCC method

CC Garber, DH Feldbruegge and M Hoessel

The method for continuous-flow assay of aspartate aminotransferase with the Technicon SMAC was modified to include preincubation of the serum enzyme with pyridoxal 5'-phosphate, to be consistent with the recommendations of IFCC and the Standards Committee of AACC. Preliminary estimates of the imprecision of the modified method on SMAC gave day-to-day standard deviations of 5.3 U/L at mean of 48 U/L (n = 66) and 6.2 U/L at 155 U/L (n = 61). Added bilirubin, sodium pyruvate, ascorbic acid, and endogenous lipids did not interfere. Comparison of results for 50 samples by this method with those by the manual IFCC method gave y = 1.1113x - 0.3 U/L, Sy/x = 4.4 U/L, and r = 0.997. Similar data are presented for the revised AST method for the DuPont aca discrete analyzer. Clinical data show that AST activities increase by as much as 200% when the serum is preincubated with pyridoxal 5'- phosphate.




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Copyright © 1981 by the American Association for Clinical Chemistry.