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Clinical Chemistry 27: 678, 1981;
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Clinical Chemistry, Vol 27, 678-678, Copyright © 1981 by American Association for Clinical Chemistry

Use of a dried blood spot in immunoreactive-trypsin assay for detection of cystic fibrosis in infants

LT Kirby, DA Applegarth, AG Davidson, LT Wong and DF Hardwick

We assayed more than 5000 blood spots dried on filter paper and approximately 1000 serum samples for immunoreactive trypsin, with commercial reagents (Behring and Sorin). The assay procedures were modified so that newborn screening is technically feasible. Both kits are satisfactory for serum assay, but the Sorin materials are better adapted for blood spot analysis. Immunoreactive trypsin in blood spots rapidly decreases with specimen age, but is stable in frozen serum. Values for premature infants do not differ significantly from those for full-term babies. Children with cystic fibrosis were readily distinguished from those without, up to at least one year of age.


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