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Clinical Chemistry 27: 753-755, 1981;
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Clinical Chemistry, Vol 27, 753-755, Copyright © 1981 by American Association for Clinical Chemistry

A kinetic standard for precise calibration of spectrophotometer cell temperature

PA Adams and MC Berman

We describe a simple, highly reproducible kinetic technique for precisely measuring temperature in spectrophotometric systems having reaction cells that are inaccessible to conventional temperature probes. The method is based on the temperature dependence of pseudo- first-order rate constants for the acid-catalyzed hydrolysis of N-o- tolyl-D-glucosylamine. Temperatures of reaction cuvette contents are measured with a precision of +/- 0.05 degrees C (1 SD).




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Copyright © 1981 by the American Association for Clinical Chemistry.