Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 27: 1205-1209, 1981;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Collet-Cassart, D.
Right arrow Articles by Masson, P. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Collet-Cassart, D.
Right arrow Articles by Masson, P. L.

Clinical Chemistry, Vol 27, 1205-1209, Copyright © 1981 by American Association for Clinical Chemistry

Automated particle-counting immunoassay for digoxin

D Collet-Cassart, CG Magnusson, CL Cambiaso, M Lesne and PL Masson

We describe a non-radioisotopic automated immunoassay for serum digoxin in the therapeutic range at 50 samples per hour by use of the particle- counting immunoassay technique and a special reagent, rheumatoid factor. Digoxin is determined by evaluating its inhibitory activity on the agglutination of digoxin-coated latex particles. The agglutinating system consists of the anti-digoxin IgG mixed with rheumatoid factor. This anti-IgG autoantibody reacts with IgG when they are aggregated or bound to a surface. Interference by serum proteins was prevented by pepsin digestion at 37 degrees C. Correlation with radioimmunoassay was r = 0.94 for 109 samples with concentrations between 0 and 5.7 micrograms/L. Analytical recovery overall averaged 102%. Within-assay precision (CV) ranged from 12.5% at 0.96 micrograms/L to 4.4% at 2.97 micrograms/L; between-assay precision varied from 8.4% at 0.75 micrograms/L to 5.8% at 2.92 micrograms/L.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1981 by the American Association for Clinical Chemistry.