Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 27: 1261-1263, 1981;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nathan, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nathan, D. M.

Clinical Chemistry, Vol 27, 1261-1263, Copyright © 1981 by American Association for Clinical Chemistry

Labile glycosylated hemoglobin contributes to hemoglobin A1 as measured by liquid chromatography or electrophoresis

DM Nathan

The utility of glycosylated hemoglobin (HbA1) measurement as an index of chronic control in diabetes can be adversely affected by interference from a labile glycosylated fraction that changes rapidly with acute changes in blood glucose concentration. I used a "high- pressure" liquid-chromatographic assay and a newly developed electrophoretic assay to quantitate the contribution of this labile fraction. If erythrocytes are incubated at 22 degrees C in isotonic saline for 12 h before hemolysis, the labile fraction is eliminated. Its contribution is similar as measured by both assays: 2-3.5% of total HbA1 in normal cells and 7-9.5% in diabetic cells. A 3-h incubation of erythrocytes with glucose produces acute changes in apparent HbA1 concentrations by both assays, but such changes can be eliminated by the incubation in saline. Although current methods are time consuming, the labile glycosylated hemoglobin must be removed when the sample is prepared for HbA1 measurement by liquid chromatography and electrophoresis if results are not to be factitiously high.


The following articles in journals at HighWire Press have cited this article:


Home page
 Diabetes CareHome page
L. N. Pani, D. M. Nathan, and R. W. Grant
Clinical Predictors of Disease Progression and Medication Initiation in Untreated Patients With Type 2 Diabetes and A1C Less Than 7%
Diabetes Care, March 1, 2008; 31(3): 386 - 390.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. PsychiatryHome page
D. C. Henderson, P. M. Copeland, T. B. Daley, C. P. Borba, C. Cather, D. D. Nguyen, P. M. Louie, A. E. Evins, O. Freudenreich, D. Hayden, et al.
A Double-Blind, Placebo-Controlled Trial of Sibutramine for Olanzapine-Associated Weight Gain
Am J Psychiatry, May 1, 2005; 162(5): 954 - 962.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J. B. Meigs, C. I. M. Panhuysen, R. H. Myers, P. W.F. Wilson, and L. A. Cupples
A Genome-Wide Scan for Loci Linked to Plasma Levels of Glucose and HbA1c in a Community-Based Sample of Caucasian Pedigrees: The Framingham Offspring Study
Diabetes, March 1, 2002; 51(3): 833 - 840.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
J. R. Galluzzi, L. A. Cupples, J. B. Meigs, P. W.F. Wilson, E. J. Schaefer, and J. M. Ordovas
Association of the Ala54-thr Polymorphism in the Intestinal Fatty Acid-Binding Protein With 2-h Postchallenge Insulin Levels in the Framingham Offspring Study
Diabetes Care, July 1, 2001; 24(7): 1161 - 1166.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
J. B. Meigs, M. A. Mittleman, D. M. Nathan, G. H. Tofler, D. E. Singer, P. M. Murphy-Sheehy, I. Lipinska, R. B. D'Agostino, and P. W. F. Wilson
Hyperinsulinemia, Hyperglycemia, and Impaired Hemostasis: The Framingham Offspring Study
JAMA, January 12, 2000; 283(2): 221 - 228.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1981 by the American Association for Clinical Chemistry.