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Clinical Chemistry 27: 1287-1290, 1981;
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Clinical Chemistry, Vol 27, 1287-1290, Copyright © 1981 by American Association for Clinical Chemistry

Plasma glucose concentrations of whole blood, as determined with a multilayer-film analytical element

A Ohkubo, S Kamei, M Yamanaka, F Arai, M Kitajima and A Kondo

A new type of multilayer film analytical element--consisting of a spreading layer, a blocking layer, an enzymic reagent layer, and a transparent layer--has been developed for blood glucose determination. After a spot of whole blood is dropped on the film, the plasma glucose concentration is determined in 6 min without further manipulations. Precise measurement of sample volume spotted on the film is not necessary for good results, nor is knowledge of the hematocrit in the range 10-46%. The minimum concentration of glucose detectable by this method is 100 mg/L. The coefficients of variation, within-run and between-run, were all less than 2.5% for 800-4000 mg/L glucose concentrations. Macromolecules and hydrophobic substances in blood did not interfere. This method seems suitable for emergency use, especially.




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Copyright © 1981 by the American Association for Clinical Chemistry.