Clinical Chemistry
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Clinical Chemistry 27: 1341-1345, 1981;
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Clinical Chemistry, Vol 27, 1341-1345, Copyright © 1981 by American Association for Clinical Chemistry

Enzyme immunoassay of antibodies to Sjogren's syndrome B antigen

AM Teppo

I describe a solid-phase enzyme immunoassay of IgG-IgA-, and IgM-type antibodies to Sjogren's syndrome B antigen. Polystyrene tubes are coated with the purified antigen. The antibodies are allowed to bind with their antigens, and then detected with alkaline-phosphatase- conjugated anti-human IgG, IgA, or IgM sera. The amount of alkaline phosphatase fixed to the tubes is determined in pH 10.0 diethanolamine buffer at 37 degrees C with p-nitrophenyl phosphate as substrate. The absorbance of the p-nitrophenolate ion liberated in 1 h at 37 degrees C is measured at 406 nm. When rheumatoid factor is present, the values obtained for IgG antibodies are too low, and those for IgM antibodies are too high.




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Copyright © 1981 by the American Association for Clinical Chemistry.