Clinical Chemistry
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Clinical Chemistry 27: 1421-1423, 1981;
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Clinical Chemistry, Vol 27, 1421-1423, Copyright © 1981 by American Association for Clinical Chemistry

Two-site immunoenzymometric assay for thyrotropin in dried blood samples on filter paper

K Miyai, K Ishibashi and M Kawashima

We describe a sensitive, simple, and rapid two-site immunoenzymometric assay for thyrotropin in dried blood samples on filter paper, for use in screening for neonatal primary hypothyroidism. In this method, two dried-blood spots of 3 mm diameter (equivalent to about 5.4 microliter of blood) are incubated overnight with anti-thyrotropin-beta-D- galactosidase complex in an anti-thyrotropin coated tube. Then the enzyme activity in the washed tube is determined fluorophotometrically. The range of thyrotropin measurable is 10 to 160 milli-int. units/L blood. Values for thyrotropin in dried blood samples determined by this method and those determined by radioimmunoassay correlated highly (r = 0.96).


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Copyright © 1981 by the American Association for Clinical Chemistry.