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Clinical Chemistry 27: 1513-1515, 1981;
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Clinical Chemistry, Vol 27, 1513-1515, Copyright © 1981 by American Association for Clinical Chemistry

Glucose oxidase immunoenzyme methodology as a substitute for fluorescence microscopy in the clinical laboratory

T Rathlev, JM Hocko, GF Franks, SC Suffin, CM O'Donnell and DD Porter

Enzymes as markers for antigens or antibodies in immunohistochemical procedures have several advantages over commonly used fluorochrome labels. These include use of a regular light microscope and the ability to get permanently stable slide preparations. Glucose oxidase (EC 1.1.2.3.4), being absent in mammalian tissue, provides no background staining, such as that seen with the commonly used horseradish peroxidase (EC 1.11.1.7) owing to peroxidase-like activity in tissues. A glucose oxidase histochemical method is detailed that is useful for detection of human antibodies; it can be easily used in clinical laboratories as a substitute for fluorescent techniques.


The following articles in journals at HighWire Press have cited this article:


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 J. Histochem. Cytochem.Home page
H. Allgayer, M. M. Heiss, R. Riesenberg, R. Babic, K. W. Jauch, and F. W. Schildberg
Immunocytochemical Phenotyping of Disseminated Tumor Cells in Bone Marrow by uPA Receptor and CK18: Investigation of Sensitivity and Specificity of an Immunogold/Alkaline Phosphatase Double Staining Protocol
J. Histochem. Cytochem., February 1, 1997; 45(2): 203 - 212.
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Copyright © 1981 by the American Association for Clinical Chemistry.