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Clinical Chemistry 27: 1586-1591, 1981;
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Clinical Chemistry, Vol 27, 1586-1591, Copyright © 1981 by American Association for Clinical Chemistry

Automated in-line ratio-correcting filter fluorometer

OS Khalil, WS Routh, K Lingenfelter, DB Carr and P Ladouceur

We have developed a novel optical arrangement for the Abbott VP bichromatic analyzer, converting it into a fluorometer upon insertion of a specially designed filter carriage. Fluorescence intensity is measured by using straight-through excitation geometry and is corrected for fluctuations in light-source intensity by using the attenuated transmitted beam through the solution as a reference. The modification allows switching from the absorbance to fluorescence mode without an auxiliary light source or a major hardware change. The detection limit for an experimental prototype was estimated as 192 pmol of fluorescein per liter. In an application to a fluorescence immunoassay for theophylline in patients' sera, the data (y) correlated well with results by an enzyme immunoassay procedure (EMIT), giving a correlation equation of y = 1.849 + 0.988x (r = 0.969). A fluorometric standard curve for glucose and some performance data are also presented.




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Copyright © 1981 by the American Association for Clinical Chemistry.