Clinical Chemistry
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Clinical Chemistry 28: 2229-2234, 1982;
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Clinical Chemistry, Vol 28, 2229-2234, Copyright © 1982 by American Association for Clinical Chemistry

Radioimmunoassay of corticotropin from plasma

J Gutkowska, J Julesz, J St-Louis and J Genest

We describe a specific and highly sensitive radioimmunoassay for corticotropin (ACTH) in human plasma. As little as 1.9 pg of circulating ACTH can be detected per milliliter (0.44 pmol/L). ACTH is first extracted from plasma samples by adsorption onto pretreated Vycor glass powder, eluted from the adsorbent by acetone, and then lyophilized. For purification of 125I-labeled ACTH, a small octadecasilyl silica column is used. The mean (and SD) concentration of ACTH in plasma from 18 ostensibly normal subjects was 10.3 (4.3) pmol/L. After overnight suppression with dexamethasone in seven of these normal subjects, their ACTH values fell to 2.62 (1.3) pmol/L (p less than 0.0005). This assay will permit clinical evaluation of ACTH plasma concentrations in clinical investigation and of the effects of drug administration on circulating ACTH.




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Copyright © 1982 by the American Association for Clinical Chemistry.