Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 28: 2264-2268, 1982;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sax, S. M.
Right arrow Articles by Silverman, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sax, S. M.
Right arrow Articles by Silverman, J. A.

Clinical Chemistry, Vol 28, 2264-2268, Copyright © 1982 by American Association for Clinical Chemistry

Liquid-chromatographic estimation of saturated phospholipid palmitate in amniotic fluid compared with a thin-layer chromatographic method for acetone-precipitated lecithin

SM Sax, JJ Moore, A Oley, JS Amenta and JA Silverman

The "high-performance" liquid-chromatographic method we report for measuring palmitate derived from saturated phospholipids in amniotic fluid is sensitive and precise and possesses a wide dynamic range. Palmitate from nonsurfactant sources is largely excluded. The procedure involves oxidation of the chloroform/methanol-extracted lipids with osmium tetroxide, precipitation of the resulting phosphatides with cold acetone, mild alkaline hydrolysis, derivatization of the liberated fatty acids to phenacyl esters, and reversed-phase chromatography. The method is no less convenient or more time-consuming than measurement of the lecithin/sphingomyelin ratio by thin-layer chromatography. The clinical usefulness of the procedure is indicated by results of a correlative study of 26 amniotic fluids evaluated by "lung profile" tests in a second laboratory. A value for dipalmitoyl lecithin of 13 mg/L or greater indicates fetal lung maturity.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1982 by the American Association for Clinical Chemistry.