Clinical Chemistry
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Clinical Chemistry 28: 2393-2397, 1982;
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Clinical Chemistry, Vol 28, 2393-2397, Copyright © 1982 by American Association for Clinical Chemistry

Radioimmunoassay for salivary estriol, with use of an 125I radioligand and a solid-phase separation technique

PL Truran, GF Read and S Walker

This simple, rapid radioimmunoassay for salivary unconjugated estriol in the third trimester of pregnancy is based on use of a Sepharose- coupled antiserum and a radioiodinated tracer, and requires only a 15- min incubation. Estriol conjugates are shown not to be present in saliva in significant concentrations; therefore a preliminary extraction is unnecessary. Determinations of unconjugated estriol in matched specimens of plasma and saliva correlate well (r = 0.95). A provisional normal range for 30 weeks to term agrees closely with those reported by others. This assay can give results quickly for the large numbers of specimens generated by weekly or daily sampling regimes.




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Copyright © 1982 by the American Association for Clinical Chemistry.