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Clinical Chemistry, Vol 28, 2418-2420, Copyright © 1982 by American Association for Clinical Chemistry
E Canalis, GE Reardon and AM Caldarella
Currently used assays for urinary cortisol reportedly overestimate it, owing to cross-reacting substances. We describe here a method for separating and measuring by liquid chromatography cortisol extracted from urine. The method is specific for cortisol and as little as 5 ng per sample can be measured. Mean analytical recovery of added cortisol was 98.8% (SD 6.1%) and the coefficients of variation ranged from 3.1 to 4.7% (within-day) and from 7.1 to 14% (between-day). Mean (and SD) urinary excretion of cortisol for 45 normal men and women was 20.1 (SD 7.6) micrograms/24 h; for 29 children it was 14.1 (SD 6.0) micrograms/24 h. Results by radioimmunoassay were 1.4- to 4.3-fold greater than by this method, and results of the two assays did not correlate well (r = 0.59, p less than 0.01). We consider the present method to be a practical and specific assay for three cortisol in urine.
The following articles in journals at HighWire Press have cited this article:
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U. Turpeinen, H. Markkanen, M. Valimaki, and U.-H. Stenman Determination of urinary free cortisol by HPLC Clin. Chem., August 1, 1997; 43(8): 1386 - 1391. [Abstract] [Full Text] [PDF]
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C.-L. Lin, T.-J. Wu, D. A. Machacek, N.-S. Jiang, and P. C. Kao Urinary Free Cortisol and Cortisone Determined by High Performance Liquid Chromatography in the Diagnosis of Cushing's Syndrome J. Clin. Endocrinol. Metab., January 1, 1997; 82(1): 151 - 155. [Abstract] [Full Text] [PDF]
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T. E. Seeman, B. S. McEwen, J. W. Rowe, and B. H. Singer Allostatic load as a marker of cumulative biological risk: MacArthur studies of successful aging PNAS, April 10, 2001; 98(8): 4770 - 4775. [Abstract] [Full Text] [PDF]
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