Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 28: 311-316, 1982;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pleban, P. A.
Right arrow Articles by Beachum, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pleban, P. A.
Right arrow Articles by Beachum, J.

Clinical Chemistry, Vol 28, 311-316, Copyright © 1982 by American Association for Clinical Chemistry

Determination of selenium concentration and glutathione peroxidase activity in plasma and erythrocytes

PA Pleban, A Munyani and J Beachum

We determined selenium concentrations and activities of the selenoenzyme, glutathione peroxidase (EC 1.11.1.9), in the plasma and erythrocytes of 38 apparently healthy women. We determined selenium concentrations directly by polarized Zeeman-effect flameless atomic absorption spectroscopy. Within-run precision studies for the assays gave CVs of 5.6% for a mean erythrocyte selenium concentration of 149.9 (SD 8.3) microgram/L (n = 10) and 6.4% for a mean plasma selenium concentration of 97.3 (SD 6.2) microgram/L (n = 12). For the women, mean selenium concentrations were 141.4 (SD 14.3) microgram/L of erythrocytes [0.49 (SD 0.07) microgram/g of hemoglobin and 96.3 (SD 14.2) microgram/L of plasma. Glutathione peroxidase activities were measured by a modification of the method of Paglia and Valentine (J. Lab. Clin. Med. 70: 158--169, 1967). Within-run precision studies for the glutathione peroxidase assays gave CVs of 12.8% for mean erythrocyte glutathione peroxidase activity of 77.2 (SD 9.9) U/g of hemoglobin (n = 13), and 8.1% for mean plasma activity of 312.5 (SD 25.2) U/L (n = 11). Mean enzyme activity was 78.7 (SD 12.9) U/g of hemoglobin for erythrocytes and 424 (SD 40) U/L for plasma. Erythrocyte selenium concentrations and glutathione peroxidase activities were positively, but poorly, correlated (r = 0.41, p less than 0.01).


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Nutr.Home page
C.-Y. O. Chen, P. E. Milbury, F. W. Collins, and J. B. Blumberg
Avenanthramides Are Bioavailable and Have Antioxidant Activity in Humans after Acute Consumption of an Enriched Mixture from Oats
J. Nutr., June 1, 2007; 137(6): 1375 - 1382.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Coll. Nutr.Home page
E. J. Ha and A. M. Smith
Plasma Selenium and Plasma and Erythrocyte Glutathione Peroxidase Activity Increase with Estrogen during the Menstrual Cycle
J. Am. Coll. Nutr., February 1, 2003; 22(1): 43 - 51.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
B. E Wendland, E. Aghdassi, C. Tam, J. Carrrier, A H. Steinhart, S. L Wolman, D. Baron, and J. P Allard
Lipid peroxidation and plasma antioxidant micronutrients in Crohn disease
Am. J. Clinical Nutrition, August 1, 2001; 74(2): 259 - 264.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Coll. Nutr.Home page
D. L. McKay, G. Perrone, H. Rasmussen, G. Dallal, W. Hartman, G. Cao, R. L. Prior, R. Roubenoff, and J. B. Blumberg
The Effects of a Multivitamin/Mineral Supplement on Micronutrient Status, Antioxidant Capacity and Cytokine Production in Healthy Older Adults Consuming a Fortified Diet
J. Am. Coll. Nutr., October 1, 2000; 19(5): 613 - 621.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
C. R Jonas, A. B Puckett, D. P Jones, D. P Griffith, E. E Szeszycki, G. F Bergman, C. E Furr, C. Tyre, J. L Carlson, J. R Galloway, et al.
Plasma antioxidant status after high-dose chemotherapy: a randomized trial of parenteral nutrition in bone marrow transplantation patients
Am. J. Clinical Nutrition, July 1, 2000; 72(1): 181 - 189.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1982 by the American Association for Clinical Chemistry.