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Clinical Chemistry 28: 467-470, 1982;
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Clinical Chemistry, Vol 28, 467-470, Copyright © 1982 by American Association for Clinical Chemistry

Enzyme immunoassay for tartrate-resistant acid phosphatase

KW Lam, M Siemens, T Sun, CY Li and LT Yam

An immunochemical method for quantitative analysis of the tartrate- resistant acid phosphatase (EC 3.1.3.2), band 5, is presented. This method involves precipitation of the enzyme from the serum by the antibody specific to band 5 and by sheep anti-rabbit immunoglobulin, followed by analysis of the enzyme activity in the precipitate. The precipitation procedure eliminates the interferences of the tartrate- sensitive phosphatase of all tissues, of the tartrate-resistant phosphatase of erythrocytes, and of unknown substances that interfere with the colorimetric method. We compare the present method with previously described colorimetric and electrophoretic methods.


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 Clin. Chem.Home page
A. J. Janckila, K. Takahashi, S. Z. Sun, and L. T. Yam
Tartrate-resistant Acid Phosphatase Isoform 5b as Serum Marker for Osteoclastic Activity
Clin. Chem., January 1, 2001; 47(1): 74 - 80.
[Abstract] [Full Text] [PDF]

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Y. R. Nakasato, A. J. Janckila, J. M. Halleen, H. K. Vaananen, S. P. Walton, and L. T. Yam
Clinical Significance of Immunoassays for Type-5 Tartrate-resistant Acid Phosphatase
Clin. Chem., December 1, 1999; 45(12): 2150 - 2157.
[Abstract] [Full Text] [PDF]


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Copyright © 1982 by the American Association for Clinical Chemistry.