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Clinical Chemistry, Vol 28, 488-495, Copyright © 1982 by American Association for Clinical Chemistry
RC Baxter, AS Brown and JR Turtle
We raised an antiserum (Tr4) in rabbits against a basic somatomedin C- like peptide preparation. Using high-immunoreactivity somatomedin C tracer, we compared the performance of radioimmunoassays in which we used the Tr4 antiserum and a well-characterized somatomedin C antiserum distributed by the National Pituitary Agency (NPA) with that of the human placental-membrane somatomedin radioreceptor assay (RRA). In their cross reactivity toward various somatomedin-like and unrelated peptides, the two radioimmunoassay methods were almost identical, although NPA antiserum, with about fourfold higher titer than Tr4 antiserum, showed a slightly greater sensitivity for most peptides tested. Radioimmunoassay of acid-ethanol-extracted plasma samples from normal persons and acromegalic, hypopituitary, hypothyroid, and renal- failure patients revealed no analytical differences between the antisera (for 122 samples, r = 0.979 between methods). Somatomedin values for acromegalic and hypopituitary samples showed no overlap with normals. Values for hypothyroid and pre-dialysis renal-failure samples were significantly lower than normal. By comparison, the RRA showed greater cross reactivity toward some somatomedin-like peptides and gave significantly lower values than radioimmunoassay for acromegalic and hypothyroid plasma extracts, and significantly higher value for hypopituitary and renal-failure samples. We conclude that the radioimmunoassay methods clearly are of greater diagnostic value than RRA for clinical somatomedin measurement.
The following articles in journals at HighWire Press have cited this article:
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  T. V. Nguyen, A. E. Nelson, C. J. Howe, M. J. Seibel, R. C. Baxter, D. J. Handelsman, R. Kazlauskas, and K. K. Ho Within-Subject Variability and Analytic Imprecision of Insulinlike Growth Factor Axis and Collagen Markers: Implications for Clinical Diagnosis and Doping Tests Clin. Chem., August 1, 2008; 54(8): 1268 - 1276. [Abstract] [Full Text] [PDF]
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 A. E. Nelson, U. Meinhardt, J. L. Hansen, I. H. Walker, G. Stone, C. J. Howe, K.-c. Leung, M. J. Seibel, R. C. Baxter, D. J. Handelsman, et al. Pharmacodynamics of Growth Hormone Abuse Biomarkers and the Influence of Gender and Testosterone: A Randomized Double-Blind Placebo-Controlled Study in Young Recreational Athletes J. Clin. Endocrinol. Metab., June 1, 2008; 93(6): 2213 - 2222. [Abstract] [Full Text] [PDF]
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 K. Sjogren, K.-C. Leung, W. Kaplan, M. Gardiner-Garden, J. Gibney, and K. K. Y. Ho Growth hormone regulation of metabolic gene expression in muscle: a microarray study in hypopituitary men Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E364 - E371. [Abstract] [Full Text] [PDF]
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A. E. Nelson, C. J. Howe, T. V. Nguyen, K.-C. Leung, G. J. Trout, M. J. Seibel, R. C. Baxter, D. J. Handelsman, R. Kazlauskas, and K. K. Ho Influence of Demographic Factors and Sport Type on Growth Hormone-Responsive Markers in Elite Athletes J. Clin. Endocrinol. Metab., November 1, 2006; 91(11): 4424 - 4432. [Abstract] [Full Text] [PDF]
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G. Johannsson, J. Gibney, T. Wolthers, K.-C. Leung, and K. K. Y. Ho Independent and Combined Effects of Testosterone and Growth Hormone on Extracellular Water in Hypopituitary Men J. Clin. Endocrinol. Metab., July 1, 2005; 90(7): 3989 - 3994. [Abstract] [Full Text] [PDF]
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R. Rej Clinical Chemistry through Clinical Chemistry: A Journal Timeline Clin. Chem., December 1, 2004; 50(12): 2415 - 2458. [Abstract] [Full Text] [PDF]
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C. Schmid, M. Brandle, C. Zwimpfer, J. Zapf, and P. Wiesli Effect of Thyroxine Replacement on Creatinine, Insulin-Like Growth Factor 1, Acid-Labile Subunit, and Vascular Endothelial Growth Factor Clin. Chem., January 1, 2004; 50(1): 228 - 231. [Full Text] [PDF]
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 C Gicquel, J Weiss, J Amiel, V Gaston, Y Le Bouc, and C D Scott Epigenetic abnormalities of the mannose-6-phosphate/IGF2 receptor gene are uncommon in human overgrowth syndromes J. Med. Genet., January 1, 2004; 41(1): e4 - 4. [Full Text] [PDF]
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A. C. F. Costa, A. Rossi, C. E. Martinelli Jr., H. R. Machado, and A. C. Moreira Assessment of Disease Activity in Treated Acromegalic Patients Using a Sensitive GH Assay: Should We Achieve Strict Normal GH Levels for a Biochemical Cure? J. Clin. Endocrinol. Metab., July 1, 2002; 87(7): 3142 - 3147. [Abstract] [Full Text] [PDF]
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R. C. Baxter, S. Meka, and S. M. Firth Molecular Distribution of IGF Binding Protein-5 in Human Serum J. Clin. Endocrinol. Metab., January 1, 2002; 87(1): 271 - 276. [Abstract] [Full Text] [PDF]
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G. Y. W. Kam, K.-C. Leung, R. C. Baxter, and Ken. K. Y. Ho Estrogens Exert Route- and Dose-Dependent Effects on Insulin-Like Growth Factor (IGF)-Binding Protein-3 and the Acid-Labile Subunit of the IGF Ternary Complex J. Clin. Endocrinol. Metab., May 1, 2000; 85(5): 1918 - 1922. [Abstract] [Full Text]
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D. M. Hoffman, R. Pallasser, M. Duncan, T. V. Nguyen, and K. K. Y. Ho How Is Whole Body Protein Turnover Perturbed in Growth Hormone-Deficient Adults? J. Clin. Endocrinol. Metab., December 1, 1998; 83(12): 4344 - 4349. [Abstract] [Full Text]
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 D. W Johnson, H. J Saunders, M. J Field, and C. A Pollock Role of insulin-like growth factor binding proteins in human post-nephrectomy proximal tubule cells J. Physiol., April 15, 1998; 508(2): 587 - 595. [Abstract] [Full Text] [PDF]
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 S. M. Shalet, A. Toogood, A. Rahim, and B. M. D. Brennan The Diagnosis of Growth Hormone Deficiency in Children and Adults Endocr. Rev., April 1, 1998; 19(2): 203 - 223. [Abstract] [Full Text]
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 R. R. Grunstein, K. Y. Ho, and C. E. Sullivan Sleep Apnea in Acromegaly Ann Intern Med, October 1, 1991; 115(7): 527 - 532. [Abstract] [PDF]
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