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Clinical Chemistry 28: 502-504, 1982;
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Clinical Chemistry, Vol 28, 502-504, Copyright © 1982 by American Association for Clinical Chemistry

Rapid quantitation of serum immunoglobulins with a miniature centrifugal analyzer

TR Koch, PK Adolf, DH Zimmerman, EC Knoblock and VM Leitz

For these quantitative turbidimetric assays for immunoglobulins IgG, IgA, and IgM in serum, the reagents used are commercially available in kit form. The two-point assays are performed with a miniature centrifugal analyzer (GEMENI, Electro-Nucleonics, Inc.). The reading taken 5 s after mixing is used to correct for specimen turbidity. Run- to-run precision (CV) was typically 5% (range: 3.0-8.7%). Excellent correlation with radial immunodiffusion was observed for each test (r greater than 0.98 for each test). The dynamic range of each test extends above and below the reference-interval ranges.




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Copyright © 1982 by the American Association for Clinical Chemistry.