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Clinical Chemistry 28: 606-608, 1982;
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Clinical Chemistry, Vol 28, 606-608, Copyright © 1982 by American Association for Clinical Chemistry

Simple, direct measurement of lipoprotein X in serum

Y Hashiguchi, M Kawaguchi, M Nakanishi, Y Matsumori, Y Kaneda, C Nakai, K Mimura and A Matsuoka

This relatively simple method for quantitative estimation of lipoprotein X is based on assay of phospholipids in precipitates of lipoprotein X. We first remove other lipoproteins by precipitation with phosphotungstic acid solution. Lipoprotein X is then precipitated from the supernatant fluid with an alkaline solution of magnesium ion, and phospholipid is determined in this precipitate. Results are linearly related to concentration of lipoprotein X. The CV is less than 3%. Results correlated well with those for agar gel electrophoresis.


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H. Fei, S. Maeda, H. Kirii, S. Fujigaki, N. Maekawa, H. Fujii, H. Wada, K. Saito, and M. Seishima
Evaluation of Two Different Homogeneous Assays for LDL-Cholesterol in Lipoprotein-X-positive Serum
Clin. Chem., September 1, 2000; 46(9): 1351 - 1356.
[Abstract] [Full Text] [PDF]


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Copyright © 1982 by the American Association for Clinical Chemistry.