Clinical Chemistry
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Clinical Chemistry 28: 1120-1124, 1982;
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Clinical Chemistry, Vol 28, 1120-1124, Copyright © 1982 by American Association for Clinical Chemistry

Measurement of plasma estradiol-17 beta by solid-phase chemiluminescence immunoassay

JB Kim, GJ Barnard, WP Collins, F Kohen, HR Lindner and Z Eshhar

We describe a simple, solid-phase chemiluminescence immunoassay for the measurement of estradiol-17 beta in extracts of peripheral venous plasma. The method has similar sensitivity, specificity, precision, and accuracy to a conventional radioimmunoassay with a tritiated antigen. An immunoglobulin G fraction od monoclonal antibodies to estradiol-6- carboyxmethyl oxime-bovine serum albumin is passively adsorbed onto the walls of polypropylene tubes. The labeled antigen is estradiol-6- carboxymethyl oxime-aminobutylethyl isoluminol. After the binding reaction (1 h at 22 degrees C), the solution is removed by aspiration (400 microliters). Sodium hydroxide (5 mol/L, 300 microliters) is added and the mixture incubated for 30 min at 37 degrees C. Luminescence is initiated by oxidation of the label with microperoxidase/hydrogen peroxide and the signal integrated for 10 s. The light yield is inversely proportional to the concentration of estradiol in the standard or sample.


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 Clin. Chem.Home page
Z. Cao, T. A. Swift, C. A. West, T. G. Rosano, and R. Rej
Immunoassay of Estradiol: Unanticipated Suppression by Unconjugated Estriol
Clin. Chem., January 1, 2004; 50(1): 160 - 165.
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Copyright © 1982 by the American Association for Clinical Chemistry.