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Clinical Chemistry 28: 1125-1128, 1982;
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Clinical Chemistry, Vol 28, 1125-1128, Copyright © 1982 by American Association for Clinical Chemistry

A colorimetric assay for releasable plasminogen activator

EE Campbell, MA Shifman, JG Lewis, JJ Pasqua and SV Pizzo

We describe an equilibrium assay for measuring release of plasminogen activator form blood-vessel walls and report data from 125 individuals free of overt thromboembolic disease. Excess human plasminogen is added to the euglobulin fraction of plasma obtained before and after venous occlusion at mean systolic pressure. To measure plasmin generation in these samples, we used the chromogenic plasmin substrate D-Val-Leu-Lys- p-nitroanilide, which liberates p-nitroaniline upon cleavage. Releasable plasminogen activator in 24 subjects was determined by this colorimetric assay and by the radiocasein assay previously reported by this laboratory (Am. J. Clin. Pathol. 76,403-409, 1981), and the results were compared. The correlation coefficient was 0.97. The colorimetric assay offers several advantages over the radiocasein assay: shorter incubation (6 vs 16 h) and no preparation or quantification of a radioactive substrate and its cleavage products.


The following articles in journals at HighWire Press have cited this article:


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 Am. J. Physiol. Endocrinol. Metab.Home page
S. Puri, D. D. Bansal, M. R. Uskokovic, and R. R. MacGregor
Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)2D3
Am J Physiol Endocrinol Metab, February 1, 2000; 278(2): E293 - E301.
[Abstract] [Full Text] [PDF]


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