Clinical Chemistry
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Clinical Chemistry 28: 1171-1173, 1982;
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Clinical Chemistry, Vol 28, 1171-1173, Copyright © 1982 by American Association for Clinical Chemistry

Rapid colorimetric assay for acetaminophen without salicylate or phenylephrine interference

MB Swanson and MI Walters

A simple rapid, and economical procedure is presented for the quantitative determination of acetaminophen in serum. Acetaminophen is extracted into solvent, and, when back-extracted into base, is simultaneously reacted with Folin-Ciocalteau reagent to form a colored complex. The potential interference of bilirubin is eliminated by treatment with p-diazobenzene sulfonic acid. Results obtained with this method agree with those obtained by "high-pressure" liquid chromatography.




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Copyright © 1982 by the American Association for Clinical Chemistry.