Clinical Chemistry
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Clinical Chemistry 28: 1204-1207, 1982;
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Clinical Chemistry, Vol 28, 1204-1207, Copyright © 1982 by American Association for Clinical Chemistry

Simplified fluorometry of total metanephrines in urine

RH Christenson, CD McGlothlin, R Hedrick and JC Cate 4th

We describe a simplified fluorometric method for quantitatively determining metanephrines in urine. The method is based on the elution of metanephrines from an ion-exchange column followed by production of a fluorescent derivative that has excitation and emission maxima of 405 nm and 505 nm, respectively. Metanephrine concentrations as great as 7.0 mg/L were within the linear range of the assay. Analytical recovery of metanephrines was 82-87%. Cross contamination by catecholamines was insignificant. The upper range of normal was 520 micrograms/24 h. Within-run and between-run CV's were 5% and about 9%, respectively. Twenty-four-hour urine specimens from five patients with pheochromocytoma showed above-normal amounts of the metanephrines by this method.




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Copyright © 1982 by the American Association for Clinical Chemistry.