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Clinical Chemistry 28: 1448-1450, 1982;
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Clinical Chemistry, Vol 28, 1448-1450, Copyright © 1982 by American Association for Clinical Chemistry

Flameless atomic absorption spectrophotometry of selenium in whole blood

RT Tulley and HP Lehmann

In this method of analysis for selenium in whole blood by flameless atomic absorption spectrophotometry, 1 mL of sample is first digested with perchloric and nitric acids. After reduction and neutralization, the sample is reacted with 2,3-diaminonaphthalene, and the product is extracted into toluene. Twenty microliters of the extract is injected into the graphite furnace of a flameless atomic absorption spectrophotometer, along with 20 microliters of a 1.0 g/L cupric nitrate solution. Blood-based standards are used to establish the standard curve. The amount of selenium required to give an absorbance of 0.0044 is 5.3 micrograms/L. Precision is good, recovery excellent. The extract is stable for 24 h.




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Copyright © 1982 by the American Association for Clinical Chemistry.