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Clinical Chemistry 28: 1742-1744, 1982;
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Clinical Chemistry, Vol 28, 1742-1744, Copyright © 1982 by American Association for Clinical Chemistry

A simple, fully enzymic bioluminescent assay for triglycerides in serum

I Bjorkhem, K Sandelin and A Thore

In this fully enzymic bioluminescent assay, triglycerides are cleaved by lipase (EC 3.1.1.3) and carboxylesterase (EC 3.1.1.1), and the glycerol obtained is phosphorylated with ATP and glycerol kinase (EC 2.7.1.30). The ATP consumed in the latter reaction is determined by the firefly luciferin-luciferase reaction, and corresponds to the concentration of triglycerides. All the enzymic reactions and the bioluminescent reading can be performed in the same test tube. The precision (CV) of the assay varied between 1 and 7% at different concentrations with a fully enzymic method based on spectrophotometry (r = 0.98).




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Copyright © 1982 by the American Association for Clinical Chemistry.