Clinical Chemistry
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Clinical Chemistry 28: 1749-1753, 1982;
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Clinical Chemistry, Vol 28, 1749-1753, Copyright © 1982 by American Association for Clinical Chemistry

Homologous radioimmunoassay for human parathyrin (residues 53-84)

W Hitzler, H Schmidt-Gayk, P Spiropoulos, F Raue and M Hufner

We describe a sequential saturation double-antibody radioimmunoassay for carboxyl-terminal fragments of human parathyrin (hPTH) in serum. Standards are prepared with synthetic hPTH (residues 53-84) in hPTH- free serum. Antisera are obtained by immunizing guinea pigs with partly purified hPTH extracted from adenomatous glands. Tracer is prepared by labeling hPTH (53-84), presumably at the histidine residue, with 125I by the Chloramine T method at pH 8.6. Dilution curves for hPTH extracted from adenomas are superimposable on dilution curves for the synthetic 53-84 fragment. Dilution of sera from hyperparathyroid patients showed linearity of response with concentration in the present assay, but non-linearity in the heterologous radioimmunoassay. In contrast to the heterologous system, which discriminated 28 of 32 patients with primary hyperparathyroidism from 32 normals (normal range: undetectable to 54 pmol/L, omitting the highest and lowest values from controls), the present assay separated these groups without overlap.




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Copyright © 1982 by the American Association for Clinical Chemistry.