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Clinical Chemistry, Vol 28, 1758-1761, Copyright © 1982 by American Association for Clinical Chemistry
K Fukuda, Y Hirai, H Yoshida, T Nakajima and T Usui
For extracting free amino acids from human leukocytes, we find that disruption of the cells by ultrasonication is more reliable than freezing and thawing. The amount of free amino acids extracted by the latter method depends on the temperature and duration of thawing. We extracted free amino acids from leukocytes of healthy men by ultrasonication and compared their concentrations in lymphocytes and granulocytes. The amino acid content of granulocytes significantly (p less than 0.001) exceeded that of lymphocytes. Of the amino acids extracted from granulocytes, 76% was taurine; for lymphocytes taurine, aspartic acid, and glutamic acid were predominant, respectively composing 44%, 17%, and 26% of the total. Taurine is proposed as an index of cell disruption.
The following articles in journals at HighWire Press have cited this article:
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  A. Kanayama and Y. Miyamoto Apoptosis triggered by phagocytosis-related oxidative stress through FLIPS down-regulation and JNK activation J. Leukoc. Biol., November 1, 2007; 82(5): 1344 - 1352. [Abstract] [Full Text] [PDF]
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 C. G. Iruloh, S. W. D'Souza, P. F. Speake, I. Crocker, W. Fergusson, P. N. Baker, C. P. Sibley, and J. D. Glazier Taurine transporter in fetal T lymphocytes and platelets: differential expression and functional activity Am J Physiol Cell Physiol, January 1, 2007; 292(1): C332 - C341. [Abstract] [Full Text] [PDF]
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 L. Doepel, M. Lessard, N. Gagnon, G. E. Lobley, J. F. Bernier, P. Dubreuil, and H. Lapierre Effect of postruminal glutamine supplementation on immune response and milk production in dairy cows. J Dairy Sci, August 1, 2006; 89(8): 3107 - 3121. [Abstract] [Full Text] [PDF]
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D. K. Emerson, M. L. McCormick, J. A. Schmidt, and C. M. Knudson Taurine Monochloramine Activates a Cell Death Pathway Involving Bax and Caspase-9 J. Biol. Chem., February 4, 2005; 280(5): 3233 - 3241. [Abstract] [Full Text] [PDF]
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R. G. Midwinter, A. V. Peskin, M. C. M. Vissers, and C. C. Winterbourn Extracellular Oxidation by Taurine Chloramine Activates ERK via the Epidermal Growth Factor Receptor J. Biol. Chem., July 30, 2004; 279(31): 32205 - 32211. [Abstract] [Full Text] [PDF]
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A. Kanayama, J.-i. Inoue, Y. Sugita-Konishi, M. Shimizu, and Y. Miyamoto Oxidation of Ikappa Balpha at Methionine 45 Is One Cause of Taurine Chloramine-induced Inhibition of NF-kappa B Activation J. Biol. Chem., June 28, 2002; 277(27): 24049 - 24056. [Abstract] [Full Text] [PDF]
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 G. E. Lobley, S. O. Hoskin, and C. J. McNeil Glutamine in Animal Science and Production J. Nutr., September 1, 2001; 131(9): 2525S - 2531. [Abstract] [Full Text] [PDF]
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