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Clinical Chemistry 28: 1772-1774, 1982;
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Clinical Chemistry, Vol 28, 1772-1774, Copyright © 1982 by American Association for Clinical Chemistry

Liquid-chromatographic determination of pentobarbital in plasma with use of a resin column and an alkaline mobile phase

RN Gupta, PT Smith and F Eng

We describe a liquid-chromatographic method involving a new, nonsilica column (XAD-2, Hamilton Co.) for pentobarbital in plasma. Plasma is extracted with chloroform after addition of the internal standard, 5- ethyl-5-p-tolyl-barbituric acid. Acidic drugs are back-extracted into alkali, then chromatographed on the resin-base reversed-phase column. The use of alkaline mobile phase allows enhanced sensitivity and detection of barbiturates at 240 nm. The within-run CV for 10 samples was 1.9%, the between-run CV 1.8%. Ten commonly used barbiturates are separated isocratically in less than 15 min. Other commonly prescribed acidic drugs do not interfere with determination of pentobarbital.




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Copyright © 1982 by the American Association for Clinical Chemistry.