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Clinical Chemistry 28: 1852-1856, 1982;
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Clinical Chemistry, Vol 28, 1852-1856, Copyright © 1982 by American Association for Clinical Chemistry

A short-term in vitro drug-screening assay for cancer chemotherapy

SL Chen, JR Snoga and H Croft

In this short-term in vitro assay for detection of tumor cell sensitivity to drugs, we have replaced the traditional soft-agar colony- forming assay with the measurement of DNA synthesis, to determine the cell renewal capability of the tumor cell population. The tumor cells are treated with drugs and cultured for five days. During the last 12-- 18 h of culture the cells are pulsed with [3H]thymidine, then harvested for scintillation counting. The effects of drugs are expressed as the percentage of DNA synthesis as compared with that of the control. When the % DNA synthesis is less than 40%, the drug is considered to be effective. So far we have studied tumor cells from multiple myeloma, non-Hodgkin's lymphoma, and carcinoma of lung, breast, ovary, stomach, and bladder. The overall negative predictive value is 1.0. The results are available within five days, compared with 21--28 days for completion of soft-agar assays; fewer cells are required; and the process is semiautomated.




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Copyright © 1982 by the American Association for Clinical Chemistry.