Clinical Chemistry
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Clinical Chemistry 28: 1873-1877, 1982;
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Clinical Chemistry, Vol 28, 1873-1877, Copyright © 1982 by American Association for Clinical Chemistry

Analysis of serum by high-field proton nuclear magnetic resonance

JL Bock

Improvements in nuclear magnetic resonance (NMR) technology are generating an expanding variety of medical applications. In this investigation I have used high-field proton NMR to identify and quantity endogenous and ingested substances in human serum. After addition of a small amount of 2H2O and a reference compound to a 0.4-mL specimen, spectra were recorded for 3 min in Fourier-transform mode, with use of presaturation to suppress the extremely intense H2O peak. Compounds detected at clinically significant concentrations include glucose, alcohols, acetone, organic acids, and salicylate. Less than 1 mmol/L of some of these substances could be detected. For serum containing 20--500 mg of added methanol per liter, peak area was a linear function of concentration (r = 0.998). High-field proton NMR, despite the drawback of expensive, sophisticated instrumentation, offers some unique advantages for clinical chemistry: it permits rapid, specific, nondestructive measurement of several compounds simultaneously, including some that may be inconvenient to measure by conventional means.


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 ANN INTERN MEDHome page
M. TRAUBE, J. L. BOCK, and J. L. BOYER
D-Lactic Acidosis After Jejunoileal Bypass: Identification of Organic Anions by Nuclear Magnetic Resonance Spectroscopy
Ann Intern Med, February 1, 1983; 98(2): 171 - 173.
[Abstract] [PDF]


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Copyright © 1982 by the American Association for Clinical Chemistry.