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Clinical Chemistry 28: 1894-1898, 1982;
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Clinical Chemistry, Vol 28, 1894-1898, Copyright © 1982 by American Association for Clinical Chemistry

Radial partition immunoassay

JL Giegel, MM Brotherton, P Cronin, M D'Aquino, S Evans, ZH Heller, WS Knight, K Krishnan and M Sheiman

In radial partition immunoassay, radial chromatography is used for performing an immunoassay. We describe the application of this technology to the measurement of digoxin in serum by enzyme immunoassay, with the entire testing procedure carried out on glass- fiber filter paper. A sample is applied to a small central area of the filter paper, where it reacts with the antibody to digoxin immobilized there. Subsequently, enzyme-labeled digoxin is applied to react with remaining antibody sites. After incubation, substrate for the enzyme is applied to the center of the reaction area and washes out any unbound label to the periphery of the paper. This step also initiates the enzyme reaction, which is quantified by front-surface fluorescence. A microprocessor-controlled automated instrument has been developed to process the filter paper matrix through the above sequence, and calculate the final result. Total testing time for digoxin is less than 7 min.


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N. B. Watts
Use of a Sensitive Thyrotropin Assay for Monitoring Treatment With Levothyroxine
Arch Intern Med, February 1, 1989; 149(2): 309 - 312.
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Copyright © 1982 by the American Association for Clinical Chemistry.