Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 29: 31-36, 1983;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lo, D. H.
Right arrow Articles by Wu, T. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lo, D. H.
Right arrow Articles by Wu, T. W.

Clinical Chemistry, Vol 29, 31-36, Copyright © 1983 by American Association for Clinical Chemistry

Assessment of the fundamental accuracy of the Jendrassik-Grof total and direct bilirubin assays

DH Lo and TW Wu

Using unconjugated bilirubin (Bu) and authentic human disconjugated bilirubin (dBc) supplemented in low-bilirubin serum pools, we have directly verified for the first time that the Jendrassik-Grof total bilirubin assay modified after Doumas et al. (Clin. Chem. 19: 984-993, 1973) detects Bu and dBc quantitatively, in Bu-equivalent concentrations relative to gravimetric concentration assignment reinforced with quantification by nuclear magnetic resonance (Wu et al., Clin. Chem. 26: 1323-1335, 1980). By contrast, the Jendrassik-Grof direct bilirubin assay (also modified after Doumas) quantifies only 70 +/- 5% of the gravimetrically determined dBc as Bu equivalents. By using Bu and dBc (instead of Bu only, as is routinely done) to calibrate the responses of the total and direct bilirubin assays, the analyses of Bu and dBc become much more quantitative. We mathematically explored the meanings of the conventional terms total, direct, and indirect bilirubins. The clinical and diagnostic implications of our observations are discussed.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
D. R. Dufour, J. A. Lott, F. S. Nolte, D. R. Gretch, R. S. Koff, and L. B. Seeff
Diagnosis and Monitoring of Hepatic Injury. I. Performance Characteristics of Laboratory Tests
Clin. Chem., December 1, 2000; 46(12): 2027 - 2049.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the American Association for Clinical Chemistry.