Clinical Chemistry
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Clinical Chemistry 29: 60-64, 1983;
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Clinical Chemistry, Vol 29, 60-64, Copyright © 1983 by American Association for Clinical Chemistry

Time-resolved fluoroimmunoassay of human choriogonadotropin

K Pettersson, H Siitari, I Hemmila, E Soini, T Lovgren, V Hanninen, P Tanner and UH Stenman

We describe time-resolved fluoroimmunoassay for human choriogonadotropin involving monoclonal antibodies directed against the beta- and alpha-subunits. The latter antibody was labeled with europium, which was measured by counting for 1 s after the immunoreaction was completed. In the solid-phase sandwich assay, both a one-step and two-step procedure were used; the respective measuring ranges were 0.7-135 and 0.7-350 int. units/L, the latter covering a 500- fold dynamic range. The CV within the assay range was between 4 and 8%, depending on the dose. Cross reactivity with lutropin in the one- and two-step procedures was 1.6% and 1.0%, respectively.


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