Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 29: 1772-1776, 1983;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eskola, J. U.
Right arrow Articles by Aho, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eskola, J. U.
Right arrow Articles by Aho, H. J.

Clinical Chemistry, Vol 29, 1772-1776, Copyright © 1983 by American Association for Clinical Chemistry

Purification and characterization of human pancreatic phospholipase A2

JU Eskola, TJ Nevalainen and HJ Aho

We purified human pancreatic phospholipase A2 from postmortem pancreatic tissue by elution of the semi-purified enzyme on CM-Sephadex C-25 with a linear NaCl gradient at pH 6.0. The enzyme appeared as a single polypeptide chain with an isoelectric point of 9.2 +/- 0.1. The relative molecular mass of the enzyme was estimated to be 15 800 +/- 1000 by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The enzyme is resistant to heating and to a 25 g/L concentration of sodium dodecyl sulfate. It is inhibited by Ca2+ ions in the presence of ovolecithin and deoxycholate. By immunohistochemical methods we showed the enzyme to be localized in the apical zymogen granule portion of pancreatic acinar cells.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Biol. Chem.Home page
W. Xu, L. Yi, Y. Feng, L. Chen, and J. Liu
Structural Insight into the Activation Mechanism of Human Pancreatic Prophospholipase A2
J. Biol. Chem., June 12, 2009; 284(24): 16659 - 16666.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
A. A. Sattar and R. Haque
Cytosolic PLA2 in Zymogen Granule Fusion and Amylase Release: Inhibition of GTP-induced Fusion by Arachidonyl Trifluoromethyl Ketone Points to cPLA2 in G-Protein-mediated Secretory Vesicle Fusion
J. Biochem., January 1, 2007; 141(1): 77 - 84.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the American Association for Clinical Chemistry.