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Clinical Chemistry 29: 1781-1786, 1983;
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Clinical Chemistry, Vol 29, 1781-1786, Copyright © 1983 by American Association for Clinical Chemistry

Free thyroxin by radioimmunoassay: evaluation of a new direct method involving a radiolabeled thyroxin analog

NP Kubasik, PA Lundberg, RG Brodows, GD Hallauer, DG Same, G Lindstedt, C Bengtsson and E Nystrom

We present here the first performance evaluation of a new direct method for free thyroxin (T4) in serum by radioimmunoassay, with use of coated tubes and a radiolabeled T4 analog (Diagnostic Products Corp.). The assay is precise and robust: within-run imprecision (CV), 3.1-6.6%; between-run imprecision, 4.0-7.9%; no demonstrable variation between technologists irrespective of experience with the method. No outliers were observed when we compared the free T4 results with serum total T4. Reference values are reported for a total of 1243 euthyroid subjects; there was no significant age effect on serum free T4 in women 26 to 72 years old. The biological variation was about +/- 35% of the mean (2 SD). Free T4 results are the same for serum and plasma. The assay performs well in hypothyroidism and hyperthyroidism, and distinguishes individuals with thyroid disease from normal individuals. Free T4 values in women taking oral contraceptives are normal. Depressed results were often observed in acute nonthyroidal illness and continuing pregnancy. These results were directly comparable with those of another commercial direct radiolabeled-T4 analog kit for free T4.




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Copyright © 1983 by the American Association for Clinical Chemistry.