Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 29: 1798-1802, 1983;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deeg, R.
Right arrow Articles by Ziegenhorn, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deeg, R.
Right arrow Articles by Ziegenhorn, J.

Clinical Chemistry, Vol 29, 1798-1802, Copyright © 1983 by American Association for Clinical Chemistry

Kinetic enzymic method for automated determination of total cholesterol in serum

R Deeg and J Ziegenhorn

We describe a rapid, kinetic, fixed-time method for determining serum total cholesterol by use of cholesterol esterase, cholesterol oxidase, and the indicator reaction with peroxidase, 4-aminophenazone, and phenol. On addition of the competitive inhibitor 3,4-dichlorophenol the Michaelis constant of cholesterol oxidase is apparently increased, which extends the linear relation between absorbance change and cholesterol concentration to 20.7 to 25.9 mmol/L, depending on the analyzer being used. For calibration, a single standard is used. Total analysis time is in the range of 80 to 210 s. Incubation temperature is 25 degrees C or 37 degrees C. The single-reagent procedure has been adapted to three different centrifugal analyzers and to the Eppendorf ACP 5040 analyzer. It yields precise and accurate results and is insensitive to potential interferences.


The following articles in journals at HighWire Press have cited this article:


Home page
 Eur Respir JHome page
J. Borgel, B. M. Sanner, A. Bittlinsky, F. Keskin, N. K. Bartels, N. Buechner, A. Huesing, L. C. Rump, and A. Mugge
Obstructive sleep apnoea and its therapy influence high-density lipoprotein cholesterol serum levels
Eur. Respir. J., January 1, 2006; 27(1): 121 - 127.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
B. I. Joffe, L. A. Distiller, and W. J. Kalk
Lipid Levels in Black South Africans With Type 2 Diabetes: Dispelling misconceptions
Diabetes Care, July 1, 2004; 27(7): 1839 - 1840.
[Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. A. van den Berg, J. D. van Buul, J. D. Ostrow, and A. K. Groen
Measurement of cholesterol gallstone growth in vitro
J. Lipid Res., February 1, 2000; 41(2): 189 - 194.
[Abstract] [Full Text]

Home page
 Am. J. Clin. Nutr.Home page
A. Pietrobelli, R. C Lee, E. Capristo, R. J Deckelbaum, and S. B Heymsfield
An independent, inverse association of high-density-lipoprotein-cholesterol concentration with nonadipose body mass
Am. J. Clinical Nutrition, April 1, 1999; 69(4): 614 - 620.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. A. van den Berg, J. D. van Buul, G. N. J. Tytgat, A. K. Groen, and J. D. Ostrow
Mucins and calcium phosphate precipitates additively stimulate cholesterol crystallization
J. Lipid Res., September 1, 1998; 39(9): 1744 - 1751.
[Abstract] [Full Text]

Home page
 NEJMHome page
G. M. Darling, J. A. Johns, P. I. McCloud, and S. R. Davis
Estrogen and Progestin Compared with Simvastatin for Hypercholesterolemia in Postmenopausal Women
N. Engl. J. Med., August 28, 1997; 337(9): 595 - 601.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the American Association for Clinical Chemistry.