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Clinical Chemistry 29: 1808-1809, 1983;
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Clinical Chemistry, Vol 29, 1808-1809, Copyright © 1983 by American Association for Clinical Chemistry

Solid-state extraction of cortisol from plasma or serum for liquid chromatography

BT Hofreiter, AC Mizera, JP Allen, AM Masi and WC Hicok

Solid-state extraction of plasma or serum offers significant advantages over traditional liquid-liquid methods in cortisol determinations by liquid chromatography (HPLC). Sample preparation is facilitated and HPLC column contamination is minimized. Extract yield from the solid- state columns is enhanced by sample pretreatment with methanol. Cortisol values by solid-state extraction/HPLC correlate well (r = 0.92) with those from a commercial radioimmunoassay procedure.




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Copyright © 1983 by the American Association for Clinical Chemistry.