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Clinical Chemistry 29: 1823-1825, 1983;
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Clinical Chemistry, Vol 29, 1823-1825, Copyright © 1983 by American Association for Clinical Chemistry

Kinetic enzymic assay for D(-)-lactate, with use of a centrifugal analyzer

CW Ludvigsen, JR Thurn, GL Pierpont and JH Eckfeldt

The D(-) isomer of lactic acid appears to cause a form of metabolic encephalopathy experienced by patients who have had jejunoileal bypass for morbid obesity. However, analysis for D(-)-lactate is not routinely available in clinical or reference laboratories. We describe an enzymic centrifugal-analyzer assay for D(-)-lactate in plasma or serum, with use of D(-)-lactate dehydrogenase. The method involves two-point kinetic calibration and preincubation of specimen and NAD+, thus eliminating the need for specimen-blanking or protein-precipitating pretreatment. This rapid, accurate, and precise assay should be helpful in evaluating patients with "short-bowel syndrome" who display confusion, lethargy, ataxia, or other central nervous-system disturbances that may be ascribable to D(-)-lactic acidosis.


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Copyright © 1983 by the American Association for Clinical Chemistry.