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Clinical Chemistry 29: 1912-1915, 1983;
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Clinical Chemistry, Vol 29, 1912-1915, Copyright © 1983 by American Association for Clinical Chemistry

DNA interference with radioimmunoassay procedures

N Matsuo and SS Koide

A bacterial factor preventing precipitation of immune complexes was purified from a culture of Streptococcus faecalis (AK strain) by gel filtration on Sephadex G-100 and Bio-Gel A-150m columns. The factor was identified as DNA on the basis of its ultraviolet absorption maximum at 260 nm, hydrolysis by deoxyribonuclease, Mr exceeding 1.5 X 10(8), and positive reaction with diphenylamine. DNA interferes with the immunoassay by preventing precipitation of immune complexes. Only double-stranded DNA and polynucleotides showed this property. Evidently, double-stranded DNA will give false-positive interference with the usual RIA procedures.




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Copyright © 1983 by the American Association for Clinical Chemistry.