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Clinical Chemistry 29: 1964-1966, 1983;
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Clinical Chemistry, Vol 29, 1964-1966, Copyright © 1983 by American Association for Clinical Chemistry

Evaluation of a solid-phase enzyme immunoassay for human choriogonadotropin beta subunit

DD Davey, MM Sample and TO Oei

We evaluated a quantitative solid-phase enzyme immunoassay for human choriogonadotropin beta subunit (beta-HCG) with anti-beta- HCG:horseradish peroxidase conjugate, recently marketed by Abbott Laboratories. We compared results on 56 patients' serum specimens, obtained mostly for followup of neoplastic disease, with those by a competitive radioimmunoassay kit. The correlation was good, the differences being of little clinical significance. Linear regression in the low and intermediate ranges gave a slope of 0.93, a y-intercept of 0.34, and a correlation coefficient of 0.97. Precision studies yielded an interassay CV of 6.4% in the intermediate range and 13% in the low range. Sensitivity was 0.69 int. unit/L. Cross reactivity was 1 to 2% with specimens fortified with lutropin or follitropin. The only substantial problem was with linearity in the upper part of the standard curve, especially in the interval, 100-200 int. units/L. This problem is obviated by adequate sample dilution.




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Copyright © 1983 by the American Association for Clinical Chemistry.