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Clinical Chemistry 29: 1969-1971, 1983;
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Clinical Chemistry, Vol 29, 1969-1971, Copyright © 1983 by American Association for Clinical Chemistry

The chemical basis and specificity of the nitrosonaphthol reaction

JA Knight, G Robertson and JT Wu

1-Nitroso-2-naphthol reacts with various 5-hydroxyindoles as well as para-substituted phenols and guaiacols. Consequently, this reaction has long been used to measure tyrosine in tyrosinosis and tyrosinemia, homovanillic acid in neuroblastoma, and 5-hydroxy-3-indoleacetic acid in the carcinoid tumor. We report here on the specificity of this reaction, as well as our early observations with respect to the chemical basis of the reaction.


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H. Zhao, J. Sagert, D. S. Hwang, and J. H. Waite
Glycosylated Hydroxytryptophan in a Mussel Adhesive Protein from Perna viridis
J. Biol. Chem., August 28, 2009; 284(35): 23344 - 23352.
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