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Clinical Chemistry 29: 1977-1980, 1983;
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Clinical Chemistry, Vol 29, 1977-1980, Copyright © 1983 by American Association for Clinical Chemistry

Assay of urinary oxalate: six methodologies compared

JE Zerwekh, E Drake, J Gregory, D Griffith, AF Hofmann, M Menon and CY Pak

To assess how well results by different methods for urinary oxalate determinations agree with each other in a clinical setting, we compared six different assays: Hodgkinson and Williams (Clin Chim Acta 36:127- 132, 1972), enzymatic, modified Hodgkinson and Williams, gas chromatography, ion chromatography, and "high-pressure" liquid chromatography. For the entire group of samples, the mean value by each method agreed relatively closely, although the enzymatic procedure produced a somewhat higher value. All six methods had large coefficients of variation within (8-58%) and between (15-88%) assays. In addition, analytical recovery by most assays was more than 100% of the added oxalate. Analytical recovery of 10 micrograms of oxalate added per milliliter of urine specimen ranged from 86 to 237%; for 20 micrograms/mL it was 83 to 320%. Thus for the six methods evaluated, no single method appeared to be superior to the others.


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 J. Am. Soc. Nephrol.Home page
G. E. von Unruh, S. Voss, T. Sauerbruch, and A. Hesse
Dependence of Oxalate Absorption on the Daily Calcium Intake
J. Am. Soc. Nephrol., June 1, 2004; 15(6): 1567 - 1573.
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Copyright © 1983 by the American Association for Clinical Chemistry.