Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 29: 2019-2021, 1983;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smith, A. M.
Right arrow Articles by Macdonald, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smith, A. M.
Right arrow Articles by Macdonald, D. J.

Clinical Chemistry, Vol 29, 2019-2021, Copyright © 1983 by American Association for Clinical Chemistry

Enzyme immunoassay for carcinoembryonic antigen

AM Smith and DJ Macdonald

In this rapid, simple enzyme immunoassay for carcinoembryonic antigen (CEA) in serum the "sandwich" system is used. The CEA is first extracted from the serum into acetate buffer by heating at 70 degrees C, then incubated in a polyvinyl microtitration plate previously coated with anti-CEA. The bound CEA is then reacted with anti-CEA conjugated to horseradish peroxidase and the activity of the peroxidase bound to the wells of the plate is quantified by adding the enzyme substrate. The assay is sensitive to 3.1 micrograms/L (which can be improved to 0.39 microgram/L by prolonging the incubation times to 120 min), precise (between-assay CV 8.0%), and shows adequate analytical recoveries. Results compared favorably with those by a radioimmunoassay kit (Abbott Diagnostics), r = 0.99. The reference interval for serum CEA in normal adults is shown. This assay is appropriate for use in monitoring patients with colorectal tumors after surgery.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the American Association for Clinical Chemistry.