| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 29, 2061-2067, Copyright © 1983 by American Association for Clinical Chemistry
S Schwartz, J Dahl, M Ellefson and D Ahlquist
We describe a new, specific, quantitative method for fecal blood, based on conversion of nonfluorescing heme to fluorescing porphyrins, that obviates serious deficiencies inherent in currently used tests. A two- reagent system is used to determine the two hemoglobin-related fractions that are found in feces. The hot citric acid extract includes only the variable fraction of porphyrins that have been preformed from heme in the intestinal tract; this often is the major fraction. Total hemoglobin is indirectly determined by reaction with heated oxalic acid:FeSO4 reagent, which converts the remaining heme to porphyrin without loss of the preformed porphyrins. A three-step purification procedure eliminates interfering materials. Analytical recovery of added hemoglobin is linearly related to concentration over a several- thousand-fold range. The assay is equally applicable to whole blood or to sub-microgram amounts of hemoglobin in the 8-mg (wet weight) fecal sample tested. Quality control by liquid chromatographic and fluorometric analysis documents fluorescence specificity of the heme- derived porphyrins.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  J. de Vogel, D. S.M.L. Jonker-Termont, E. M.M. van Lieshout, M. B. Katan, and R. van der Meer Green vegetables, red meat and colon cancer: chlorophyll prevents the cytotoxic and hyperproliferative effects of haem in rat colon Carcinogenesis, February 1, 2005; 26(2): 387 - 393. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Carrier, E. Aghdassi, J. Cullen, and J. P. Allard Iron Supplementation Increases Disease Activity and Vitamin E Ameliorates the Effect in Rats with Dextran Sulfate Sodium-Induced Colitis J. Nutr., October 1, 2002; 132(10): 3146 - 3150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C. Harewood, J. P. McConnell, J. J. Harrington, D. W. Mahoney, and D. A. Ahlquist Detection of Occult Upper Gastrointestinal Tract Bleeding: Performance Differences in Fecal Occult Blood Tests Mayo Clin. Proc., January 1, 2002; 77(1): 23 - 28. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Jiang, J. M. Jeter, S. E. Nelson, and E. E. Ziegler Intestinal Blood Loss During Cow Milk Feeding in Older Infants: Quantitative Measurements Arch Pediatr Adolesc Med, July 1, 2000; 154(7): 673 - 678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. K Lund, S G. Wharf, S. J Fairweather-Tait, and I. T Johnson Oral ferrous sulfate supplements increase the free radical–generating capacity of feces from healthy volunteers Am. J. Clinical Nutrition, February 1, 1999; 69(2): 250 - 255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Bhisutthibhan, X.-Q. Pan, P. A. Hossler, D. J. Walker, C. A. Yowell, J. Carlton, J. B. Dame, and S. R. Meshnick The Plasmodium falciparum Translationally Controlled Tumor Protein Homolog and Its Reaction with the Antimalarial Drug Artemisinin J. Biol. Chem., June 26, 1998; 273(26): 16192 - 16198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Yip, P. J. Limburg, D. A. Ahlquist, H. A. Carpenter, A. O'Neill, D. Kruse, S. Stitham, B. D. Gold, E. W. Gunter, A. C. Looker, et al. Pervasive Occult Gastrointestinal Bleeding in an Alaska Native Population With Prevalent Iron Deficiency: Role of Helicobacter pylori Gastritis JAMA, April 9, 1997; 277(14): 1135 - 1139. [Abstract] [PDF]
|
|
|
|
 |
|
 J. L. Blackshear, V. S. Baker, A. Holland, S. C. Litin, D. A. Ahlquist, R. G. Hart, R. Ellefson, and J. Koehler Fecal Hemoglobin Excretion in Elderly Patients With Atrial Fibrillation: Combined Aspirin and Low-Dose Warfarin vs Conventional Warfarin Therapy Arch Intern Med, March 25, 1996; 156(6): 658 - 660. [Abstract] [PDF]
|
|
|
|
|
|
G. P. Young and D. J. B. St. John Screening for Colorectal Cancer: The Accuracy of Fecal Occult Blood JAMA, July 28, 1993; 270(4): 451 - 452. [Abstract] [PDF]
|
|
|
|
|
|
D. A. Ahlquist, H. S. Wieand, C. G. Moertel, D. B. McGill, C. L. Loprinzi, M. J. O'Connell, J. A. Mailliard, J. B. Gerstner, K. Pandya, and R. D. Ellefson Accuracy of Fecal Occult Blood Screening for Colorectal Neoplasia: A Prospective Study Using Hemoccult and HemoQuant Tests JAMA, March 10, 1993; 269(10): 1262 - 1267. [Abstract] [PDF]
|
|
|
|
 |
|
 D. J. B. St. John, G. P. Young, J. G. McHutchison, M. C. Deacon, and M. A. Alexeyeff Comparison of the Specificity and Sensitivity of Hemoccult and HemoQuant in Screening for Colorectal Neoplasia Ann Intern Med, September 1, 1992; 117(5): 376 - 382. [Abstract] [PDF]
|
|
|
|
|
|
D. A. Ahlquist, G. G. Klee, D. B. McGill, and R. D. Ellefson Colorectal Cancer Detection in the Practice Setting: Impact of Fecal Blood Testing Arch Intern Med, May 1, 1990; 150(5): 1041 - 1045. [Abstract] [PDF]
|
|
|
|
|
|
K. K. Knight, J. E. Fielding, and R. N. Battista Occult Blood Screening for Colorectal Cancer JAMA, January 27, 1989; 261(4): 586 - 593. [Abstract] [PDF]
|
|
|
|
|
|
D. A. AHLQUIST, S. SCHWARTZ, J. ISAACSON, and M. ELLEFSON A Stool Collection Device: The First Step in Occult Blood Testing Ann Intern Med, April 1, 1988; 108(4): 609 - 612. [Abstract] [PDF]
|
|
|
|
 |
|
 M. P. Miller and T. V. Stanley Results of a Mass Screening Program for Colorectal Cancer Arch Surg, January 1, 1988; 123(1): 63 - 65. [Abstract] [PDF]
|
|
|
|
|
|
D. A. AHLQUIST, D. B. McGILL, S. SCHWARTZ, W. F. TAYLOR, M. ELLEFSON, and R. A. OWEN HemoQuant, A New Quantitative Assay for Fecal Hemoglobin: Comparison with Hemoccult Ann Intern Med, September 1, 1984; 101(3): 297 - 302. [Abstract] [PDF]
|
|
|
|
|
|
J. G. STEWART, D. A. AHLQUIST, D. B. McGILL, D. M. ILSTRUP, S. SCHWARTZ, and R. A. OWEN Gastrointestinal Blood Loss and Anemia in Runners Ann Intern Med, June 1, 1984; 100(6): 843 - 845. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
