Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 29: 2103-2105, 1983;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neeley, W. E.
Right arrow Articles by Zettner, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neeley, W. E.
Right arrow Articles by Zettner, A.

Clinical Chemistry, Vol 29, 2103-2105, Copyright © 1983 by American Association for Clinical Chemistry

Multilayer film analysis for glucose in 1-microL samples of plasma

WE Neeley and A Zettner

With the ultramicroanalytical system described here we can measure glucose in 1 microL of plasma or serum. The sample is placed on a dry, multilayer film element (Eastman Kodak), where a colored spot about 3.5 mm in diameter develops. The reflectance of these spots is measured with a reflectance digital matrix photometer that was conceived, designed, and constructed in our laboratory. The spot is illuminated with monochromatic light and its image is projected by a camera lens onto the photosensitive surface of a linear photodiode array containing 512 individual photodetectors. The photodetector signals are processed by a computer to obtain the reflectance and diameter of the spot. The latter is proportional to sample volume. Because the reflectance of the spot does not depend greatly on sample volume, accurate pipetting is not required. The coefficients of variation of repeatable glucose analyses were, for 400, 3000, and 5120 mg/L, 1.7, 2.3, and 2.8%, respectively. The correlation coefficient (r) between glucose analyses by our method (y) and with the Ektachem 400 (x) was 0.9918; the regression equation was y = 1.07x - 94.3 mg/L.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the American Association for Clinical Chemistry.