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Clinical Chemistry 29: 225-236, 1983;
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Clinical Chemistry, Vol 29, 225-236, Copyright © 1983 by American Association for Clinical Chemistry

Diagnostic efficacy of newer synthetic-substrates methods for assessing coagulation variables: a critical overview

J Fareed, HL Messmore, JM Walenga and EW Bermes Jr

With the introduction of synthetic substrates, many newer methods have been developed for testing coagulation parameters. Some of these methods are used in clinical settings, and many have been evaluated for their diagnostic efficacy in various coagulation disorders. Clinical chemistry laboratory instrumentation has been used to accommodate these methods. Commercially available kits for antithrombin III, heparin, plasminogen, alpha 2-antiplasmin, and factor X are used by many laboratories. Individual reagents have also become available and are being used to develop assays for routine diagnostic use. The development of suitable standards is rapidly underway, and their availability will facilitate adoption of these methods by hospital laboratories. Many of the synthetic-substrate-based assays that have been introduced for routine diagnostic purposes have been published recently. Here, we provide an overview of the newer amidolytic methods for the measurement of certain coagulation variables.




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Copyright © 1983 by the American Association for Clinical Chemistry.